Dr. Mauro Luisetto
Previous Europeans specialist laboratory medicine EC4 REGISTERED, Italy
Title: m R.N.A. purification : TECHNOLOGY ASPECTS and impurities TFF, Chromatography, UF/DF ( Resins, magnetic beads , monoliths )
Abstract:
m
R.N.A. VACCINE production like other biopharmaceutical need various
purification stages in the manifacturing
process.
In
this process are used technique like : T.F.F., tangential flow filtration followed by different chromatografic
procedure ( affinity and ion exchange separation ) with various kinds of resin an then UF/DF
technique. ( ultrafiltration -diafiltration).
So
it is of interest to verify the material used for this kind of procedure as solid phases or for the membrane and if released impurity
in the final product.
For Silica-based Reversed-Phase packings, a
carbon load percentage indicates the amount of functional bonded phase attached
to the Silica-base material.
Aim
of this work is to investigate the role played by this characteristics in the
separation process of m R.N.A.
Because this
parameter influence the retention time it is interesting to evaluate
the use in separation technique of biophramaceutictals.
And
this also for the carbon coated silica
colums .
Silica
gel for chromatography can be produced by systetic process bu alsto form Rice
treated at high temperature .
What
kind of effect can be played using an high level of carbon coated silica material
on the final purified product ?
The
graphitic particle can be finded as
impurity during the manifacturing process of this resins ?
And
waht is the role played by carbon (
graphene- quantum dots) membrane as
reported in various research application?
Biography:
Applied
pharmacologist, hospital pharmacist manager, professorship general toxicology
Professor
of environmental toxicology and pharmacology, Previous Europeans specialist
laboratory medicine EC4 REGISTERED since 2019.
Many
publications and books in scientific discipline : pharmacology , pharmacy ,
toxicology, pathology , neuroscience, virology , epidemiology, management , ICT
RESEARCH
INTEREST SCORE ON RESEARCHGATE : MORE THEN 99% OF ALL RG MEMBERS
IMA
academy Marijnskaya Italy Applied
chemistry and industrial chemistry branch.